ImpedanCELL (SF4206 - ICORE)

Platform accredited at national level by GIS IBiSA

Contact

The team

• Director: Dr Christophe DENOYELLE

The platform draws on the expertise and know-how of the following people to meet users' needs:

• Dr Christophe DENOYELLE, Platform Director and Scientific Manager at the Baclesse site;
• Dr Stéphane PRONOST, Deputy Director of the LABÉO research division and scientific manager on the LABÉO site;
• Dr Emilie BROTIN, Platform Research Engineer and Technical Manager at the Baclesse site;
• Dr Erika HUE, research engineer and technical manager at the LABÉO site;
• Ms Christine FORTIER, technical manager of the Normandie Equine Vallée platform (Saint-Contest site) and quality relay, Research Division, LABÉO site;
• Dr Laurent POULAIN, Deputy Director of UMR Inserm 1086 Anticipe and head of the BioTICLA laboratory;
• Dr Romain PAILLOT, Head of Equine Health, LABÉO Research Unit.

Activities

ImpedanCELL is an innovative and original platform designed to meet the needs of any user wishing to study the dynamic monitoring of cellular behavior in real time, including at high throughput. ImpedanCELL is open to local and national collaborations and services, both academic and industrial, and offers training courses for users of impedancemetry (xCELLigence technology) and real-time cell imaging (IncuCyte S3) systems.

Created in 2012, the ImpedanCELL high-throughput real-time cell activity measurement platform was integrated into the technical platforms of Structure Fédérative 4206 ICORE (Interactions Cellules, ORganismes, Environnement) at the Université de Caen Normandie in 2014. It has been pre-labeled by the GIS IBiSA (Infrastructures en Biologie Santé et Agronomie) since January 1, 2019.

It is spread over two technical sites:

• Center de lutte contre le cancer (CLCC) François Baclesse for all non-infectious applications
• LABÉO for all virology and bacteriology applications (confined room L2).

At the Centre François Baclesse, it is housed in a dedicated room in the Research building, within the BioTICLA laboratory (Biology and Innovative Therapies for Ovarian Cancers), which is one of the two thematic axes of UMR Inserm 1086 Anticipe (Interdisciplinary Research Unit for Cancer Prevention and Treatment).

On the LABÉO site, it is hosted on the Normandie Equine Vallée platform (Saint Contest site), which houses LABÉO researchers, the BIOTARGEN team (Biology, Genetics and OsteoArticular and Respiratory Therapies - EA7450) (EA7450) from the University of Caen Normandie.

The range of applications we have developed includes the study of adhesion, proliferation, viability, cytotoxicity and differentiation, as well as changes in cell morphology and the functional analysis of receptors (GPCR, RTK), with direct applications in numerous fields such as oncology, neuroscience, immunology, toxicology, bacteriology, virology and marine biology.

Technical resources

The ImpedanCELL platform enables real-time, high-throughput monitoring of cellular activity using two technologies: impedance measurement (xCELLigence) and cellular imaging (IncuCyte S3).

Thanks to the support of the Centre François Baclesse, LABÉO, the Université de Caen Normandie, the Normandy region, the French government and European funds (FEDER), the platform is equipped with high-performance equipment (two xCELLigence MP systems, each with 6 96-well plates, and one xCELLigence DP system with 3 16-well plates) enabling the study of adhesion, proliferation, cell death, migration, invasion and more. Applications in fields as varied as oncology, neuroscience, immunology or marine biology are feasible.

A third xCELLigence MP system with 6 96-well plates is available at LABÉO in a confined L2 laboratory for microbiology experiments.

In 2017 and 2020, the platform acquired three real-time cell imaging systems (IncuCyte S3) distributed across each of the two sites. This equipment automates image acquisition and analysis in long-term kinetics (visible and fluorescence), and enables the study of 6 96-well plates simultaneously directly from an incubator.

The ImpedanCELL platform is open to local and national collaborations and services, both academic and industrial. It is involved in training initiatives at the University of Caen Normandie's IUT and UFR Santé and Sciences, thanks to a mobile iCELLigence system that can be used outside the platform. It also offers practical training for users of impedancemetry and cellular imaging systems, and carries out all or part of the analyses on a contract basis, according to user requirements.

Major publications

1. Florent R, Weiswald LB, Lambert B, Brotin E, Abeilard E, Louis MH, Babin G, Poulain L, N'Diaye M. Bim, Puma and Noxa upregulation by Naftopidil sensitizes ovarian cancer to the BH3-mimetic ABT-737 and the MEK inhibitor Trametinib. Cell Death Dis. 2020 May 18;11(5):380. PMID: 32424251.
2. Vernon M, Lambert B, Meryet-Figuière M, Brotin E, Weiswald LB, Paysant H, Vigneron N, Wambecke A, Abeilard E, Giffard F, Louis MH, Blanc-Fournier C, Gauduchon P, Poulain L, Denoyelle C. Functional miRNA screening identifies wide-ranging antitumor properties of miR-3622b-5p and reveals a new therapeutic combination strategy in ovarian tumor organoids. Mol Cancer Ther. 2020 May 5: molcanther.0510.2019. PMID: 32371581.
3. Lepleux C, Marie-Brasset A, Temelie M, Boulanger M, Brotin E, Goldring MB, Hirtz C, Varès G, Nakajima T, Saintigny Y, Savu D, Chevalier F. Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes. J Cell Commun Signal. 2019 Sep;13(3):343-356. PMID: 30903603.
4. Thieulent CJ, Hue ES, Fortier CI, Dallemagne P, Zientara S, Munier-Lehmann H, Hans A, Fortier GD, Pitel PH, Vidalain PO, Pronost SL. Screening and evaluation of antiviral compounds against Equid alpha-herpesviruses using an impedance-based cellular assay. Virology. 2019 Jan 2;526:105-116. PMID: 30388626.
5. Hedir S, De Giorgi M, Fogha J, De Pascale M, Weiswald LB, Brotin E, Marekha B, Denoyelle C, Denis C, Suzanne P, Gautier F, Juin P, Ligat L, Lopez F, Carlier L, Legay R, Bureau R, Rault S, Poulain L, Oliveira Santos JS, Voisin-Chiret AS. Structure-guided design of pyridoclax derivatives based on Noxa / Mcl-1 interaction mode. Eur J Med Chem. 2018 Nov 5;159:357-380. PMID: 30308410.
6. Sauvage F, Fattal E, Al-Shaer W, Denis S, Brotin E, Denoyelle C, Blanc-Fournier C, Toussaint B, Messaoudi S, Alami M, Barratt G, Vergnaud-Gauduchon J. Antitumor activity of nanoliposomes encapsulating the novobiocin analog 6BrCaQ in a triple-negative breast cancer model in mice. Cancer Lett. 2018 Sep 28;432:103-111. PMID: 29883750.

Partners

At regional level :

• Academic research teams (CEA, CNRS, EA, IFREMER, Inserm) from Structure Fédérative 4206 ICORE, Université de Caen Normandie and Université de Rouen, and industrial partners.

At national level :

• Academic research teams (Cergy-Pontoise, Chatenay Malabry, Grenoble, Nantes, Paris, Toulouse, Rennes) and industrial partners.

Internationally :

• Academic research teams (Argentina, Great Britain).