SéSAME (Séquençage pour la Santé, l'Agronomie, la Mer et l'Environnement - Sequencing for Health, Agriculture, the Sea and the Environment), a new-generation sequencing platform developed with the Université de Caen Normandie and located at the Centre François Baclesse, in the Cancer Biology and Genetics Laboratory.
Contact
Activities
The aim of the SéSAME platform is to provide local teams with premises, equipment and technical and bioinformatics support for high-throughput sequencing projects in the fields of health, agronomy, the sea and the environment.
In cancer diagnostics and research, the platform is used to study hereditary forms of breast and ovarian cancer, and to identify molecular targets for tumor therapy.
The methods developed within the platform include :
- Target-seq (reseq including exome),
- DNA-seq (whole genome),
- RNA-seq (transcriptome),
- sRNA-seq (miRNA-seq),
- Capture-C.
The platform's bioinformatics services include :
- Genotyping (mutation search, insertion/deletion),
- Search for variations in the number of copies,
- Differential expression,
- Differential splicing,
- Assembling small genomes.
Technical resources
The platform is equipped with :
- 2 Illumina sequencers, a Nextseq500 and a NextSeq2000
- 2 STAR "High-Throughput" robots (Hamilton)
- 1 covaris E220 sound level meter
- 1 tapestation 2200 (Agilent)
- 1 QIASymphony nucleic acid extraction machine (Qiagen)
- 1 3500 capillary sequencer (thermofisher)
- 1 dedicated computing farm and storage servers for bioinformatics (576 cores, 3,000 GB of memory and 200 TB of storage)
Partnerships
- Regional: Université de Caen Normandie, Laboratoire LABEO, CHU de Rouen, Inserm U1245