Platform for Research and Innovation in Mass Spectrometry and Metabolism (PRISMM)
Contact
The team
- Department Head: Pr Raphaël DELEPEE
- Teacher-researchers :
- Raphaël DELEPEE, Professor, Quantitative Bioanalysis, sample preparation
- Claire LOPEZ-PIFFET, Senior Lecturer, Metabolomics
- Guillaume SAINT-LORANT, Hospital Practitioner, Quality Manager
- Marc SINCE, Senior Lecturer, Pharmacokinetics and drug metabolism
- Technical staff :
- Stéphanie Lagadu, Outstanding Teaching and Research Technician: sample preparation, LC-MS/MS analysis
- Isabelle Vaudorne, Technical assistant, Prevention officer: sample preparation, semi-preparative HPLC purification
Activities
PRISMM (Plateforme de Recherche et d'Innovation en Spectrométrie de Masse et Métabolomique) is a service, research and training platform focused primarily on the identification and quantification of new biomarkers. It offers the analysis and quantification of small molecules with a molecular weight of less than 2000 Da from a wide variety of samples, using innovative analytical methods with or without mass spectrometry. The platform's expertise applies to the identification, analysis and assay of small molecules using UHPLC-MS/MS, UHPLC-UV and HPLC-UV.
The platform's skills include the preparation of complex samples by solid-phase, liquid/liquid and printed polymer extraction. Small quantities of compounds of interest can also be purified from complex mixtures. Our mastery of extraction techniques enables us to analyze biomarkers in any type of matrix, whether related to health-related issues (serum, red blood cells, whole blood, urine, brain, cerebrospinal fluid, etc.) or to the environment (water, sediments, fish, molluscs, cephalopods, etc.). Large series of analyses can be performed. PRISMM has nationally and internationally recognized expertise in the analysis of DNA bases modified by exposure to genotoxic compounds (DNA adducts), as well as in the analysis of neurotransmitters and their metabolites.
Depending on the case, analyses can be carried out in the form of simple services, extended services for the development and validation of new analysis methods, or in the form of research partnerships.
Technical resources
- Triple quadrupole mass spectrometer (UHPLC-UV-ESI-MS/MS, Shimadzu 8030Plus, 2012)
- Separative and semi-preparative system (UHPLC-Diode array, Shimadzu Nexera X2, 2018)
- Solid phase extraction equipment (single and high throughput, 2020)
- Access to a UPLC-IMS-HRMS/MS system dedicated to metabolomics (Waters Vion, 2019)
Partners
At regional level :
- Academic teams in the fields of oncology, neuroscience and the environment (UMR INSERM, UMR CNRS and EA).
At national level :
- Academic laboratories in French Guiana, Brittany and Occitania (IFREMER, CNRS, INRA and IRD).
Internationally :
- Academic laboratories in Norway, Lebanon and Morocco.