Lower Normandy SéSAME (sequencing for health, agronomy, the sea and the environment) new generation sequencing platform, developed with the University of Caen, Normandy and located within the Centre François Baclesse, in the biology and cancer genetics laboratory.
The aim of the SéSAME platform is to provide regional research teams with premises, equipment and technical and bioinformatics support for high-throughput sequencing projects in the fields of health, agronomy, the sea and the environment.
In oncology, in the fields of diagnosis and research, the platform is used to study hereditary forms of breast and ovarian cancer, and to identify molecular targets of therapeutic interest in tumours.
Methods developed within the platform include:
- Target-seq (reseq including exome)
- DNA-seq (whole genome)
- RNA-seq (transcriptome)
- sRNA-seq (miRNA-seq)
The platform’s bioinformatics services include:
- Genotyping (search for mutations, insertion/deletion)
- Research into variations in the number of copies
- Differential expression
- Differential splicing
- Small genome assembly
The platform is equipped with:
- 2 Illumina sequencers, 1 Nextseq500 and 1 NextSeq2000
- 2 ‘High-Throughput’ robots STAR (Hamilton)
- 1 E220 Covaris ultrasonicator
- 1 2200 TapeStation (Agilent)
- 1 QIASymphony (Qiagen) nucleic acid extraction automaton
- 1 3500 capillary sequencer (Thermofisher)
- 1 dedicated render farm and storage servers for bioinformatics (576 cores, 3,000 Gb of memory and 200 Tb of storage)
- At regional level: University of Caen Normandy, Laboratoire LABEO, CHU university hospital in Rouen, Inserm U1245